The effect of storage temperature is practically one of the most important factors
affecting maintenance of efficacy of B.C.G. vaccine. This investigation was conducted in
five different temperatures of our seasons in Korea; the Summer season with maximum
temperature of 32℃, the Winter season mean temperature of-10℃, the Spring and
Autumn of about 20t℃ and the control temperatures of 37℃ as in tropic areas and 2℃
between 5℃ as in refrigerated storage temperature. The harmful effects of storage on
the viability of the liquid B.C.G. vaccine were observed. In this experiment 20 million
viable units of B.C.G. vaccine 1㎎ per c.c. prepared at B.C.G. laboratory, The National
Institute of Health, Seoul, Korea were used. In this experiment we observed the
following :
1. In a storage temperature of -10℃, after 60days viable units of 990,000 per c.c. on
viable B.C.G. colony were confirmed.
2. In a storage temperature of 2℃-5℃, after 30 days the viable units were confirmed
to be 8,280,000 and after 60 days 580,000 per c.c. in B.C.G. vaccine.
As the harmful effect of storage under warm temperature on liquid B.C.G. vaccine
was confirmed in this experiment, precaution should be taken, at all times, to maintain
the B.C.G. vaccine under 2℃ between 5℃, temperatures during transport and storage.
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